This instrument consists of Multi-Photon Laser Scanning Confocal Microscope (Olympus FV1000) with a Fluorescence Lifetime Imaging Attachment (Becker & Hickl). It is among the most advanced, commercially-available optical imaging systems, and gives CINT a world-class capability for optical characterization of any array of biological, synthetic, and hybrid nanomaterials.

Capabilities include:

• Fluorescence Recovery After Photobleaching (FRAP).
• Fluorescence Resonance Energy Transfer (FRET).
• Total Internal Reflectance Fluorescence (TIRF).
• FLIA module enables spatial mapping of fluorescence lifetimes. 
• MCP-PMT detector allows for lifetime resolution imaging in near-IR.

Contact: George Bachand

Research Highlight:
Formation of lipid nanotube networks through kine-driven extrusion of multicomponent, phase-separated giant unilamellar vesicles
Imam, Z. I.; Bachand, G. D. Langmuir 2019, 35 (49), 16281–16289. doi:10.1021/acs.langmuir.9b02637