A super-resolution microscope based upon single molecule detection and localization (e.g. PALM, STORM, or d-STORM), including both acquisition and analysis software.

Capabilities include:

• Dual color emission detection.
• Polarization-resolved detection.
• Single molecule localizations down to a few nm.
• Through-objective Total Internal Reflection (TIR) excitation.
• Highly Inclined Laminated Optical Sheet (HILO) excitation.

Technical Specifications:

•  Excitation wavelengths: 405 nm, 488 nm, 532 nm, 632 nm.
•  Various emission filters for above excitation wavelengths.
•  Built upon Olympus IX 71 microscope base.
•  Exploits two Princeton Instruments Pro-EM CCD cameras.
•  Uses custom auto-focus feedback system to minimize Z-drift.

Contacts:
Jim Werner
Dean Morales

Research Highlights:
A Framework for Quantitative Analysis of Spectral Data in Two Channels
Ryan, D. P.; Dunlap, M. K.; Majumder, S.; Werner, J. H.; Hollingsworth, J. A.; Gelfand, M. P.; Van Orden, A.; Goodwin, P. M. Applied Physics Letters 2020, 117 (2), 024101. doi.org/10.1016/j.actamat.2022.117794

A Gain Series Method for Accurate EMCCD Calibration
Ryan, D. P.; Dunlap, M. K.; Gelfand, M. P.; Werner, J. H.; Van Orden, A. K.; Goodwin, P. M. Scientific Reports 2021, 11 (1). doi.org/10.1038/s41598-021-97759-6